Moffitt Cancer Center


From November 2009-December 2011, Danielle conducted immunology research in the lab of Dr. Pearlie Epling-Burnette at Moffitt Cancer Center.  During her time in the lab, Danielle assisted in determining the mechanism of action of an immunomodulatory drug, lenalidomide,  used to treat Myelodysplastic Syndrome (MDS).  She also studied the cellular regulation of Protein Phosphatase 2A (PP2A) in response to DNA damage and lenalidomide as a potential inhibitor of PP2A.  During the academic year, she worked 10-15 hours a week.  In 2011, Danielle was nominated for the Barry M. Goldwater Scholarship, a national scholarship for students pursuing research careers within science, technology, math, or engineering.

Additionally, Danielle participated in Moffitt Cancer Center’s Summer Program for the Advancement of Research Knowledge (SPARK) during the summer of 2010 and 2011.  Danielle worked 40-60 hours a week.

In-vitro techniques performed include: Western Blot, immunoprecipitation, cell culture, cell transfection, flow cytometry (cell cycle analysis), Enzyme-linked immunosorbent assay (ELISA), bacteria culture and plasmid isolation, Malachite Green phosphatase assay, and Bradford protein assay. 

In-vivo techniques performed include: mouse dissection, intraperitoneal injection and spleen and lymph node tissue isolation.

 


Danielle presenting at the 2011 National Conference
on Undergraduate Research in Ithaca, New York

Danielle with Dr. Epling-Burrnette and graduate student, Jessica, receiving the Excellence in Undergraduate Research Award from USF Honors College Dean 


Danielle in the Epling-Burnette laboratory


2010 SPARK Research Day at Moffitt Cancer Center




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Danielle Grams,
Sep 8, 2012, 9:26 PM
ĉ
Danielle Grams,
Sep 8, 2012, 9:26 PM
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